lysing of hypha

David Hagerberg mik_daha at luecology.ecol.lu.sexx
Mon Apr 21 10:24:13 EST 1997


Chris Wright wrote:
> 
> If you are going for a DNA extraction from a pure culture, why don't you
> lyophilize and grind the hyphae in liquid nitrogen?  There are numerous
> protocols for DNA extraction which will take it from there.
> Thanks Chris,

but do you do if you had treated these creatures that bad, and they still 
do not give any DNA in extraction?

>                       Good luck,
>                                     Chris
> 
> David Hagerberg <mik_daha at luecology.ecol.lu.sexx> wrote:
> >Hello!
> >
> >I'm going to purify DNA out of hyphi from an extremely troublesome
> >fungus, Drechmeria coniospora. Nothing seems to work. Now, I want to
> >degrade the hyphal cell walls before lysing the hyphi. Do anyone have a
> >protocoll for cell wall degradation that leaves the hyphi intact or is it
> >better to lyse the hyphi at same time as the cell wall?
> >
> >Best Regards



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