How to get a Clean Spore-Print?
Biologische.Pilze at t-online.de
Fri Aug 29 16:26:32 EST 1997
As a real Novice concerning starting cultivation of shrooms from Spores lately
tried propagation of our own grown oyster-spores, as a first step (began
with oysters because I thought oyster to be the simplest to try as they are well
known to be comparably quick growing...) that I got from a rather fresh
specimen from a 'comparably-clean' spawn-run-room where for incidence a
*Cut off a piece of the cap-underside using scissors and tweezers and attached
them using some grease under the top of a clean agar plate and kept them off any
medium for half an hour...(hoped to get most contaminants fallen off already)
*Then put them on a clean MYPA-medium and twisted the top every 1.5 hours a bit
so that after one day the whole dish was really loaded with spore-prints
=> Was Optically-Real-Good-Lookin! :-)
But bad result: Only bacterial infections, and virtually NOT a single spot (on
10 parallel dishes!!) where I had the impression it could be worth propagation
of the growing visual mycelium to another dish for clean-up steps... 8-(
Who has Experience and likes to share them with us?
Is it generally possible in Routinous Work without any Antibiotics? -> How??
We are a small Organic Mushroom Farm and would like to avoid any Chemical Agens
if possible (although I obtained a small set of Antibiotics...) because I fear
it is sometimes in routine-work unevitable to use it...
Out of this very first trial and error would think it could be (from the
Organic-Point-of-View) the Absolute Clean Solution to come through to get a
confirmed pure-culture (mycelial-growth) first, propagate it sterily and get a
fruiting in an as clean as possible environment (say within a sterilised
Erlenmayer-flask) and use the so-grown-specimen to get a real clean spore-sprint
But this meant by far the most Time-Consuming-Way-to-Grow!?, Thomas
Thomas Ziegler, Biologische.Pilze at t-online.de, D-97892 Unterwittbach, Germany
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