Sterile GLASS Petri Dishes
rpeter at nmsu.edu
Thu Feb 6 12:43:47 EST 1997
Having done it both ways, I much prefer dry heat. Unless you
drop the vacuum fast,you get condensate on the glass from
pressure cooker sterilization.
There are several things to think about in choosing glass over
plastic. In my lab we use plastic for almost everything because
it turns out to be cheaper by quite a lot because:
glass dishes cost an arm and a leg
they break easily (especially tops) so you have to buy lots of
extra tops. As one of my friends obeserved, you only need so
many potted plant saucers (which is what we who do plant microbe
work wind up using excess bottoms for)
They have to be washed very clean before re use. Even paying a
minimum wage dish washer it costs me more to use glass.
On the other hand, if you want to grow stuff a long time, glass
often holds the cultures longer without drying. They use less
land fill space (but more wash water) than plastic and may be
cheaper IF you don't count your time in the cost.
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