Quick and dirty PCR

Elisa Becker EBECKER at UVVM.UVIC.CA
Thu Feb 27 21:44:15 EST 1997


I was wondering if you could give me advice/references for doing quick and
dirty PCR using species-specific primers on either mycelium on agar plates
or infected wood samples.  I am hoping for a simple procedure like grinding and
or boiling the tissue in an extraction buffer (with ProteinaseK or BME maybe?),
spinning the tissue down, and diluting it for use in the reaction.
Thanks in advance for your help!
Elisa



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