Quick and dirty PCR

Kelly Patrice Collins kpcollin at students.wisc.edu
Wed Mar 5 06:57:28 EST 1997


>For dried (or fresh) specimens, just take a little bit of the gill tissue 
>and grind it up in 500 ul of TBE, spin it down for 10 minutes at about 
>6k.  The supernatant can be used in PCR straight, or more successfully, 
>at a 1:100 dilution.  


Sorry!! That's 500 ul of TE, not TBE, for anyone questioning that 
statement.  The hand is quicker than the mind.......




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