Alan S. Wicks
NAP-4 at worldnet.att.net
Thu Oct 9 18:37:16 EST 1997
C M Manoj wrote:
> Dear Sirs,
> We need some help. If someone could come forward with some
> guidelines we will be much grateful.
> We are dealing with Oyster Mushrooms. We are facing with problems
> from infection. One typical problem is like this.
> We are doing the tissue culture (pre mother spawn) in the media made
> up of
> Potato essence, dextrose and Agar. We are doing the surface steri-
> lization of the jars/testubes using Hgcl2 and then clean the same in
> steriized water. But instead of white growth we are getting some thing
> n green/black(mostly green). Is this a problem with
> inoculation/sterilization ? Is this actually a viral infection ?
> This is not the end of our problems. We are having problems with
> next stages also. Which I can explain if someone could come forward
> with some guidelines/help.
> Thanx in advance,
> CM Manoj &Suresh
Try using some Hydrogen Peroxide or Household Bleach (1:6 v/v) to
sterilize your glassware, etc. Also, Rush Wayne, who is sometimes
around this group, has a handbook for sale on using hydrogen peroxide in
growing media that helps reduce contamination - it works. Also, you
might try 20 grams agar, 20 grams maltose (the light stuff which you can
get at any beer making supply store) and 2 grams of yeast extract per
liter of water.
Alan S. Wicks
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