growing mycelium in a liquid culture from tissue

Chris Wright Wrightc2 at
Sat Jul 4 15:26:27 EST 1998

Growing mycelium in a liquid medium is not difficult (providing that it
is a fungus which is culturable). I would suggest isolating the culture
on an agar medium first to insure you are working with a (putative) pure
culture.  Then use the same formula you used in the agar medium except
hold the agar.  Grow the mycelium in sterile erlenmyer flasks which have
a foam plug or some type gas exchange apparatus.  Do not fill the flasks
too full.  There is a tradeoff between surface area and amount of
substrate.  Mycelium can be grown either static or shaken.

Good luck,


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