Ustilago or Phytophthora protoplasts

[Arnaud Bottin]

To prepare Ustilago or Phytophthora protoplasts, lysing enzymes from
Trichoderma harzianum ("Novozym 234") work usually well. They are only sold
by Sigma but this company stops the production. Do you know about
alternative enzymes ? Thank you for your reply.


Dr Arnaud Bottin
UMR 5546 CNRS - UPS			Phone +33 (0)5 621 935 18
Pôle de Biotechnologie Végétale		FAX   +33 (0)5 621 935 25 
24, Chemin de Borde-Rouge		Mél   bottin at cict.fr
BP 17
Auzeville
31326 Castanet-Tolosan
FRANCE