water agar

[Trevor D'Souza]

Hi:

Water agar:

20 g Agar in 1 liter distilled water

Mix reagents.

For test-tubes:
Bring to a boil.
Dispense 15-ml aliquots into 25 x 125 mm test tubes.
Autoclave for 15 min at 15 psi.

For agar plates:
Autoclave for 15 min at 15 psi.
Molten agar medium cooled to 45 C and poured into sterile Petri dishes
prior to use.

Water agar plates are generally used to induce sporulation in fungi.

Trevor


<**********************>
Trevor M. D'Souza, Ph.D.
Research Associate
Department of Microbiology
Michigan State University
East Lansing, MI 48824-1101.
<**********************>


HYUN C KIM wrote:

> Hi, I read a few articles on the nematophagous fungi, and have came
> across a few mentiong about the "water agar" which is believed to be
> used in counting infected nematodes by nematophagous destroying fungi.
> can anyone who knows about this please tell me what this "water agar"
> supposed use? or even its composition? Any lead to this clue will be
> much appriciated! thank you!