Methods to transfer a plasmid into Pisolithus tinctorius wanted!
xiaomar at hotmail.com
Fri Apr 20 07:56:45 EST 2001
I am a student working on my undergraduate thesis now. My project is to
transfer a plasmid( about 11Kb) into the fungi Pisolithus tinctorius. The
method I use now is to acquire the protoplast from the mycelium, incubate
the protoplast with the plastmid, and then transfer the plasmid into
protoplast by electroporation.
But I have some difficulties in getting the protoplast of Piolithus
tinctorius. Could you tell me the methods?
Or, do you have any other methods to transfer a plasmid into Piolithus
Thank you very much!
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