[Mycology] Quantification of nematophagous fungi

[Branislav]

Hi Peter,

The most reliable way (but not the cheapest) is to isolate and purify soil DNA 
sample, and using  A. oligospora - specific set of primers (ITS) run a 
quantitative PCR. I bet you can find a bunch of protocols searching PubMed. 
Good luck.

Bran


On Monday 27 November 2006 02:07, wachira peter wrote:
> I am currently working on biological control of nematodes in the soil using
> the nematophagous fungi. To do this, i have to quantify the nemtophagous
> fungi in the soil before planting and after the maturity of my test crop.
> could somebody kindly assist me with protocol of quantification of these
> fungi. I will be intersted with Arthrobotrys oligospora.
>
>   Thank you.
>
>   Peter.
>
>
> ---------------------------------
> Everyone is raving about the all-new Yahoo! Mail beta.