[Mycology] PCR primers for fungi identification

[WU Yucheng]

Dear members,

Recently I isolated several fungi from soil but the identification is 
really hard ---- the morphological method is too sophisticated to a novice 
like me. I notice that ribosomal DNA sequencing based method is widely-used 
in papers, which is relative simple and convenient. However, the primers 
used by different groups seem to be diverse, which may target either 18S 
ribosomal or ITS sequences. I try to looking for some reviews or articles, 
but which seems to be rare. So, may anybody can tell me which primers 
should be choosed, and how about the reliability of phylogenetic 
relationship speculated from DNA sequence?

Thanks very much in advanced!

==================================================== 
Yucheng Wu
Ph.D. Student
Soil and Envirionment Bioremediation Research Center(SEBC) 
Institute of Soil Science, Chinese Academy of Sciences
71 East Beijing Road, Nanjing, China
yucheng_wu from hotmail.com
====================================================

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