[Mycology] Culturing Rhizopus or other Zygomycota
Fred M. Rhoades
(by fmrhoades from comcast.net)
Wed Dec 12 14:34:14 EST 2007
I have a set of questions that I have been wondering about for many years.
What do folks do for presenting the Zygomycota to introductory
biology college students?
Which culture source(s) work best? (which genus and which supply
firm) Anyone use Mucor?
Which agar medium?
For many years we have been depending on biological supply house
cultures of mating strains (+/-) of Rhizopus stolonifer. On PDA,
these cultures always produce an overabundance of asexual sporangia
and the zygosporangia are mixed in with the aerial hyphae and asexual
sporangia, not arranged in a tidy row between the two strains (as I
remember from my first mycology course long ago - but maybe that
wasn't Rhizopus). We have tried Phycomyces but the asexual
sporangium production is skimpy, and the protective antler-like
structures surrounding the zygosporangia interfere with true
understanding of the sexual reproductive process. Our lab preparator
has expressed some concern about the use of Mucor, because of its
association with respiratory diseases.
Fred M. Rhoades, Biology
Western Washington University
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