[Mycology] (no subject)

[mfk]

Mycos ha scritto:
> I was wondering what the trick had to have been! Many years ago Paul 
> Kroeger picked up a second hand one from the UBC auction for a mere 
> $100. Needless to say, both he and I were pleased as punch considering 
> the filter alone was worth more than that --- nevertheless an assembled, 
> ready-to-go LFH.
> 
> And they are indeed the cat's meow for culture work, but surprisingly 
> not as commonplace as one would expect. For instance, some time ago I 
> went up to the local college here in Kamloops to do some private work 
> which they had graciously loaned their facilities to me for. When I got 
> there however I find they were using their fume hoods as a kind of 
> poor-man's glove-box.
> 
> Apart from my embarrassment at having to decline the offer (I decided 
> the cost of my specimens being lost to contamination wasn't worth the 
> risk of me being gracious and accepting the circumstances) was the 
> curious matter of a mycologist being the acting Dean of the 
> Science/Forestry wing at the time. I would have thought that LFHs would 
> have been his first order of business! <g> Oh, well. The social-sciences 
> need their specimens as well <g>.
> 
> Gary Williams
> 
> Ken Hoover wrote:
>> In <3qvls2$m6r at ixnews3.ix.netcom.com> jimcald at ix.netcom.com (James
>> Caldwell) writes:
>>> I just finished an article for a beer magazine on how to build a
>>> laminar flow hood (for about 100 bucks).  Since I got the idea
>>> originally from Stamet's book on mushroom culturing, it occurred to me
>>> that some readers of this group might be interested as well.  I'll fax
>>> or snail mail copies to any interested party.
>>
>>
>> Wow!   I grossly underestimated the interest in this article; I got
>> more than 20 requests and 24hours haven't even elapsed.  I have been
>> E-mailing a text version of the article to those who have requested it.
>>  If you desire the article with photos and simple drawings (not really
>> necessary IMHO) please send me a SASE at
>>
>> Jim Caldwell
>> 9180 Nana Russell Rd.
>> Owings, MD  20736
>>
>> (I'll spring for the postage for non-US addresses if you send a
>> self-addressed envelope)
>>
>> In the meantime, requests for the article text or questions can be
>> directed to me at jimcald at ix.netcom.com
>>
>> For the curious, the key to my design was the use of an inexpensive
>> high output (520 cfm) radial output fan with back-curved impellers, and
>> downsizing the HEPA filter to 12" x 24".  Going to a larger filter
>> (which the fan will handle) would up the cost from about $100 to $140
>> or so.  Sources for the above parts are included in the article.
>>
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>>
> 

I can understand you! However gloveboxes work perfectly, and in case of 
failure you can always clean the culture with a couple of transfers 
(that are necessary in a glovebox if molds are present, as you know).

A lot of people understimate the power of a cheap glovebox made of a 
common plastic container with 2 holes.

However flowhood is a must, to work more confortable and on a higher 
rate of success.

I've read of people that clone mushrooms in loco using a disposable 
plastic bag with 2 holes for the hand and a spray like alcohol.

Fabio - www.FreeSporePrints.org