[Mycology] Conservation of root samples before staining?

[Rasheed Adeleke]

Just store the root in 50% ethanol, I have done this several times and it worked. Wash the ethanol off the root samples before staining.

Deborah Renz <deborah.renz At unibas.ch> wrote:  Hello

I would like to know if it is possible to conserve fresh roots after 
plant harvest for a longer time (several weeks to month) before they are 
stained to determine %root colonization by AMF. In what a solution 
should I put the roots?

Or is it possible the stain oven-dried roots?

(My plant species are Plantago lanceolata, P. media, Hieracium pilosella 
& Prunella grandiflora; we jused calcareous grassland soil as AMF Inoculum).


Thanks & cheers!
Deborah

-- 


Deborah Renz

Department of Environmental Sciences
Section of Conservation Biology
University of Basel

St. Johanns-Vorstadt 10
CH-4056 Basel, Switzerland

e-mail deborah.renz At unibas.ch
tel ++41// (0)61/ 267 08 42
fax ++41// (0)61/ 267 08 32

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Adeleke Rasheed 
Department of Biochemistry, 
Microbiology and Biotechnology,
Rhodes University,
Grahamstown, 6140
South Africa.





 
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