[Mycology] Re: Mycology Digest, Vol 19, Issue 4

[mehdi zarei]

you can remove fine roots in each individual sample  , rinse with tap water and place in 15ml falcon-tube with standard Formalin-Aceto-Alcohol (FAA  with ratio of 90:1:1) fixing solution for measuring the percent of root colonization  and have them for a longer time . you can  find this article as follows:
  1.     Kormanik, P. P., and McGraw, A. C., 1982. Quantification of vesicular-arbuscular mycorrhizae in plant roots. In: Schenck NC [ed.] Methods and principles of mycorrhizal research. American Phytopathological Society, St. P aul, pp.37-45.
   
  All the best
  Mehdi Zarei
  Soil Ecology Department of UFZ; Halle, Germany
   
  
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Today's Topics:

1. Conservation of root samples before staining? (Deborah Renz)
2. Re: Conservation of root samples before staining?
(Rasheed Adeleke)


----------------------------------------------------------------------

Message: 1
Date: Mon, 29 Jan 2007 20:10:48 +0100
From: Deborah Renz 
Subject: [Mycology] Conservation of root samples before staining?
To: mycology At magpie.bio.indiana.edu
Message-ID: <45BE46B8.5070403 At unibas.ch>
Content-Type: text/plain; charset=ISO-8859-15; format=flowed

Hello

I would like to know if it is possible to conserve fresh roots after 
plant harvest for a longer time (several weeks to month) before they are 
stained to determine %root colonization by AMF. In what a solution 
should I put the roots?

Or is it possible the stain oven-dried roots?

(My plant species are Plantago lanceolata, P. media, Hieracium pilosella 
& Prunella grandiflora; we jused calcareous grassland soil as AMF Inoculum).


Thanks & cheers!
Deborah

-- 


Deborah Renz

Department of Environmental Sciences
Section of Conservation Biology
University of Basel

St. Johanns-Vorstadt 10
CH-4056 Basel, Switzerland

e-mail deborah.renz At unibas.ch
tel ++41// (0)61/ 267 08 42
fax ++41// (0)61/ 267 08 32



------------------------------

Message: 2
Date: Mon, 29 Jan 2007 14:28:29 -0800 (PST)
From: Rasheed Adeleke 
Subject: Re: [Mycology] Conservation of root samples before staining?
To: Mycology At magpie.bio.indiana.edu
Message-ID: <567823.37716.qm At web34401.mail.mud.yahoo.com>
Content-Type: text/plain; charset="iso-8859-1"

Just store the root in 50% ethanol, I have done this several times and it worked. Wash the ethanol off the root samples before staining.

Deborah Renz wrote: Hello

I would like to know if it is possible to conserve fresh roots after 
plant harvest for a longer time (several weeks to month) before they are 
stained to determine %root colonization by AMF. In what a solution 
should I put the roots?

Or is it possible the stain oven-dried roots?

(My plant species are Plantago lanceolata, P. media, Hieracium pilosella 
& Prunella grandiflora; we jused calcareous grassland soil as AMF Inoculum).


Thanks & cheers!
Deborah

-- 


Deborah Renz

Department of Environmental Sciences
Section of Conservation Biology
University of Basel

St. Johanns-Vorstadt 10
CH-4056 Basel, Switzerland

e-mail deborah.renz At unibas.ch
tel ++41// (0)61/ 267 08 42
fax ++41// (0)61/ 267 08 32

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Adeleke Rasheed 
Department of Biochemistry, 
Microbiology and Biotechnology,
Rhodes University,
Grahamstown, 6140
South Africa.






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