[Mycology] RE: Mycology Digest, Vol 27, Issue 3

Geis, Phil via mycology%40net.bio.net (by geis.pa from pg.com)
Thu Sep 13 12:31:23 EST 2007


Though conidia can be produced, you likely want to understand the
presence of growing viable mycelial elements that would express
nematophagy.
Think about it - what would be the value of plate counts or MPN of a
mycelial fungus?  A colony could be one or one thousand cells and an
increase in apparent cfu could mean more conidia not necessarily more
mycelium.  Same goes for MPN sequential dilutions - and that's presuming
you'd be able to differentiate the specific fungus from all the others.

For isolation, suggest you read the article :
http://ibj.pasteur.ac.ir/July2004/Shams-b.htm

Quantitative PCR is unlikely to help in this context much.  You'd not
know copy number or that the fungi were alive or recently deceased.  You
might consider culture baiting (see above) in a spatial sense.


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Subject: Mycology Digest, Vol 27, Issue 3

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Today's Topics:

   1. Quantification of nematophagous fungi (wachira peter)
   2. Mushroom ID, (#1) Mycology Digest, Vol 27, Issue 2 (Ken Hoover)


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Message: 1
Date: Tue, 11 Sep 2007 21:14:28 -0700 (PDT)
From: wachira peter <wachirapm from yahoo.com>
Subject: [Mycology] Quantification of nematophagous fungi
To: Mycology from magpie.bio.indiana.edu
Message-ID: <443270.87878.qm from web35405.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I am a PhD student working with nematophagous fungi  isolated from soil
in vegatable gardens.
  Could somebody kindly explain to me how i could quantify the
nematophagous fungi from the soil so that i could monitor their
population change. 
  Is there a selective media that i could use to isolate and quantify
Arthrobotrys oligospora?
  Kindly also explain to me the MPN procedure as it has been used by
some authors.
   
  Thank you.
   
  Peter.


Peter M. Wachira: BSc, MSc (University of Nairobi)
University of Nairobi
BGBD PROJECT
Admin. Assistant
       
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Message: 2
Date: Wed, 12 Sep 2007 14:21:51 -0500
From: "Ken Hoover" <kent.hoover from gmail.com>
Subject: [Mycology] Mushroom ID, (#1) Mycology Digest, Vol 27, Issue 2
To: mycology from oat.bio.indiana.edu
Message-ID:
	<1fb7b21a0709121221rd0c7cf1oec63080e56582bf0 from mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Probably an Omphalotus species with a lighter orange coloration.
Perhaps O.
olivascens?    I've read that O. illudens and O. olivascens have been
both
grouped as variants of the same species, O. olearius.

W/O a spore print and information regarding bruising/staining, it is
difficult to difinitively place this mushroom.


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