From pxc215 from hotmail.com Thu Sep 11 13:32:56 2008 From: pxc215 from hotmail.com (pxc215@hotmail.com) Date: Thu Sep 11 16:39:34 2008 Subject: [Mycology] Graduate Assistantship in Fungal Systematics & Evolution Message-ID: The Department of Plant Sciences and Landscape Architecture, University of Maryland, College Park, is seeking a M.Sc. or Ph.D. student to start in the Fall 2009. The student will study the systematics and evolution of either Cosmospora or Neonectria (Nectriaceae, Ascomycota). These genera are common tropical and temperate fungi and some of them are important plant pathogens and potential agents of biocontrol. Fieldwork and laboratory work are important components of this research. The student will be supported by the prestigious NSF’s Partnerships for Enhancing Expertise in Taxonomy program (PEET). The PEET project is entitled: “Monographic studies in the Nectriaceae, Hypocreales: Nectria, Neonectria, and Cosmospora.” Drs. Priscila Chaverri (UMD), Amy Y. Rossman (ARS-USDA), and Gary J. Samuels (ARS-USDA) are the Principal Investigators in this project. The student will be supported for two years for a M.Sc. and 3-4 years for a Ph.D. and it includes stipend (about $20,000/year) and tuition, plus supplies and domestic and international travel. To apply, please send a complete Curriculum vita, contact information, names and contact information of 2-3 referees, and a one-page cover letter stating the applicant’s qualifications, background and reasons for applying. Review of applications will start September 30, 2008 and will continue until the position is filled. The deadline to apply to University of Maryland is February 1, 2009 and all the University's admission requirements are detailed at www.gradschool.umd.edu. Send requested materials to: Dr. Priscila Chaverri Assistant Professor Department of Plant Sciences and Landscape Architecture 2112 Plant Sciences Building University of Maryland College Park, Maryland 20742 pchaverr@umd.edu From hardus.hatting from fabi.up.ac.za Tue Sep 16 08:20:33 2008 From: hardus.hatting from fabi.up.ac.za (hardus.hatting@fabi.up.ac.za) Date: Tue Sep 16 09:18:59 2008 Subject: [Mycology] asking for help Message-ID: <48CFCEC1.28524.960AE5A2@hardus.hatting.fabi.up.ac.za> Hi Paul I am battling with Bacillus bacterial contamination in autoclaved wheat and rice at the moment. I need to get the wheat/rice mixture 100% sterilized, but I think that this specific strain might be heat resistant. At the moment I am autoclaving the wheat/rice mixture at 121?C for 30 min. I will then incubate the flasks with the autoclaved wheat/rice mixture for 24 hours and will repeat the autoclaving again after 24 hours. This I will repeat three times, but to my disappointment it seems that the bacteria are not killed and after 1-2 weeks I will still loose allot of wheat/rice cultures due to bacterial contamination. Regarding the autoclave works 100% 1) Is the 121?C temperature sufficient to kill the Bacillus? 2) Is the autoclaving time cycle of 30 min. sufficient? 3) How long must the autoclaving cycles be apart to make sure you kill the bacterial spores.? 4) Is there any other methods I can use to kill the bacterial spores? I will really appreciate it if you can give me a few tips of how to get rid of this bacterial problem. Thanks allot Hardus From prakashpy123 from yahoo.co.in Tue Sep 16 23:16:59 2008 From: prakashpy123 from yahoo.co.in (Prakash Peralam Yegneswaran) Date: Wed Sep 17 12:32:53 2008 Subject: [Mycology] Re: Mycology Digest, Vol 39, Issue 2 In-Reply-To: <200809161704.m8GH4EV20291@net.bio.net> Message-ID: <217884.79917.qm@web8902.mail.in.yahoo.com> Please Find the answers as below the each questions ? 1) Is the 121?C temperature sufficient to kill the Bacillus? Bacillus sps are notorious in forming endospores. Probably I think you might have encountered a thermophilic sps. It is sufficient to kill the vegetative form but not the resistant endospores 2) Is the autoclaving time cycle of 30 min. sufficient? ?Sufficient 3) How long must the autoclaving cycles be apart to make sure you kill the bacterial spores.? ? 12 hrs 4) Is there any other methods I can use to kill the bacterial spores? ? Autoclaving for 3 consequtive days with 12 hrs difference will help better in getting rid of the spores. ? PRAKASH PERALAM YEGNESWARAN Faculty of Medicine, Lecturer in Microbiology, Incharge Medical Mycology Laboratory, Department of Microbiology, II Floor, Centre for Basic Sciences, Kasturba Medical College, Manipal University, Madhav Nagar, Manipal, Karnataka State, INDIA. ----------------------- Pincode : 576 104 Tel Off. : +91-820 2571201 extn. 22322 Mobile : +91-9886616153 Fax : +91-820 2571927 Email : prakashpy123@yahoo.co.in --- On Tue, 16/9/08, mycology-request@oat.bio.indiana.edu wrote: From: mycology-request@oat.bio.indiana.edu Subject: Mycology Digest, Vol 39, Issue 2 To: mycology@magpie.bio.indiana.edu Date: Tuesday, 16 September, 2008, 10:34 PM Send Mycology mailing list submissions to mycology@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/mycology or, via email, send a message with subject or body 'help' to mycology-request@net.bio.net You can reach the person managing the list at mycology-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Mycology digest..." Today's Topics: 1. asking for help (hardus.hatting@fabi.up.ac.za) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Sep 2008 15:20:33 +0200 From: hardus.hatting@fabi.up.ac.za Subject: [Mycology] asking for help To: "mycology@iubio.bio.indiana.edu" Cc: hardus.hatting@fabi.up.ac.za Message-ID: <48CFCEC1.28524.960AE5A2@hardus.hatting.fabi.up.ac.za> Content-Type: text/plain; charset=ISO-8859-1 Hi Paul I am battling with Bacillus bacterial contamination in autoclaved wheat and rice at the moment. I need to get the wheat/rice mixture 100% sterilized, but I think that this specific strain might be heat resistant. At the moment I am autoclaving the wheat/rice mixture at 121?C for 30 min. I will then incubate the flasks with the autoclaved wheat/rice mixture for 24 hours and will repeat the autoclaving again after 24 hours. This I will repeat three times, but to my disappointment it seems that the bacteria are not killed and after 1-2 weeks I will still loose allot of wheat/rice cultures due to bacterial contamination. Regarding the autoclave works 100% 1) Is the 121?C temperature sufficient to kill the Bacillus? 2) Is the autoclaving time cycle of 30 min. sufficient? 3) How long must the autoclaving cycles be apart to make sure you kill the bacterial spores.? 4) Is there any other methods I can use to kill the bacterial spores? I will really appreciate it if you can give me a few tips of how to get rid of this bacterial problem. Thanks allot Hardus ------------------------------ _______________________________________________ Mycology mailing list Mycology@net.bio.net http://www.bio.net/biomail/listinfo/mycology End of Mycology Digest, Vol 39, Issue 2 *************************************** Be the first one to try the new Messenger 9 Beta! Go to http://in.messenger.yahoo.com/win/ From geis.pa from pg.com Wed Sep 17 06:20:07 2008 From: geis.pa from pg.com (Geis, Phil) Date: Wed Sep 17 12:33:14 2008 Subject: [Mycology] RE: Mycology Digest, Vol 39, Issue 2 In-Reply-To: <200809161704.m8GH4GV20298@net.bio.net> References: <200809161704.m8GH4GV20298@net.bio.net> Message-ID: <3B4A11B0E75E524AB9EFDF2BDC5CE4F801A76D01@bdc-emb005.na.pg.com> You could try gamma irradiation. -----Original Message----- From: mycology-bounces@oat.bio.indiana.edu [mailto:mycology-bounces@oat.bio.indiana.edu] On Behalf Of mycology-request@oat.bio.indiana.edu Sent: Tuesday, September 16, 2008 1:04 PM To: mycology@magpie.bio.indiana.edu Subject: Mycology Digest, Vol 39, Issue 2 Send Mycology mailing list submissions to mycology@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/mycology or, via email, send a message with subject or body 'help' to mycology-request@net.bio.net You can reach the person managing the list at mycology-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Mycology digest..." Today's Topics: 1. asking for help (hardus.hatting@fabi.up.ac.za) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Sep 2008 15:20:33 +0200 From: hardus.hatting@fabi.up.ac.za Subject: [Mycology] asking for help To: "mycology@iubio.bio.indiana.edu" Cc: hardus.hatting@fabi.up.ac.za Message-ID: <48CFCEC1.28524.960AE5A2@hardus.hatting.fabi.up.ac.za> Content-Type: text/plain; charset=ISO-8859-1 Hi Paul I am battling with Bacillus bacterial contamination in autoclaved wheat and rice at the moment. I need to get the wheat/rice mixture 100% sterilized, but I think that this specific strain might be heat resistant. At the moment I am autoclaving the wheat/rice mixture at 121?C for 30 min. I will then incubate the flasks with the autoclaved wheat/rice mixture for 24 hours and will repeat the autoclaving again after 24 hours. This I will repeat three times, but to my disappointment it seems that the bacteria are not killed and after 1-2 weeks I will still loose allot of wheat/rice cultures due to bacterial contamination. Regarding the autoclave works 100% 1) Is the 121?C temperature sufficient to kill the Bacillus? 2) Is the autoclaving time cycle of 30 min. sufficient? 3) How long must the autoclaving cycles be apart to make sure you kill the bacterial spores.? 4) Is there any other methods I can use to kill the bacterial spores? I will really appreciate it if you can give me a few tips of how to get rid of this bacterial problem. Thanks allot Hardus ------------------------------ _______________________________________________ Mycology mailing list Mycology@net.bio.net http://www.bio.net/biomail/listinfo/mycology End of Mycology Digest, Vol 39, Issue 2 *************************************** From newmy51 from gmail.com Thu Sep 18 17:32:12 2008 From: newmy51 from gmail.com (Danny Newman) Date: Thu Sep 18 21:36:57 2008 Subject: [Mycology] ABC Oyster Cultivation Message-ID: <24a95ed90809181532i7511bbb4se8e8502180ddb5af@mail.gmail.com> Going from memory of a friend's highly simplified explanation of mushroom cultivation, I've taken pieces of a local ostreatus fruit and placed them between the slots of a bunch of strips of corrugated cardboard, soaked and rolled them and left them in a cool dry place for about a week. Not a whole lot of myceliation so far. I know the tissue is more than viable for cloning, but without any real cultivation equipment readily available, is this a decent method for starting an oyster patch? Is there an alternate, preferable method? Additionally, would coffee grounds alone suffice as a substrate? Any and all help is appreciated. Thanks in advance, -Danny From dwheeler from ipns.com Sun Sep 21 03:22:34 2008 From: dwheeler from ipns.com (dwheeler@ipns.com) Date: Sun Sep 21 13:05:45 2008 Subject: [Mycology] Re: ABC Oyster Cultivation References: Message-ID: <3d99d22a-cb4f-45b5-bcc7-6ab77e45c3b3@r15g2000prh.googlegroups.com> On Sep 18, 3:32 pm, "Danny Newman" wrote: > Going from memory of a friend's highly simplified explanation of mushroom > cultivation, I've taken pieces of a local ostreatus fruit and placed them > between the slots of a bunch of strips of corrugated cardboard, soaked and > rolled them and left them in a cool dry place for about a week. Not a whole > lot of myceliation so far. I know the tissue is more than viable for > cloning, but without any real cultivation equipment readily available, is > this a decent method for starting an oyster patch? Is there an alternate, > preferable method? Additionally, would coffee grounds alone suffice as a > substrate? Any and all help is appreciated. > > Thanks in advance, > > -Danny If you have taken portions of the gills including spores (and I don't see any way you couldn't) then you will have mycelium develop. Pleurotus ostreatus likes warm conditions for growing: optimal conditions are 77 degrees F. and near 90% humidity. If you haven't placed the cardboard inside a plastic bag, do so ASAP. The preferred method is to take a small portion of the stipe that has not previously been exposed to air, and place it on fresh agar, then incubate in a warm area to see if you can get a pure culture. Your method will work, but it requires optimal conditions for P.o. to grow, rather than the estimated 10,000 spores contained in each breath you take. In other words, it can work. But only time will prove if it actually does work now. Sealing the substrate inside plastic removes a lot of possible contaminants from your attempted culture. Paul Stamets has used fresh coffee grounds to grow Pleurotus ostreatus on. And it works. You can also soak a roll of paper towels in 2 cups of warm water, add pieces of fresh Pleurotus ostreatus, and seal in a large plastic bag. You can even use fresh newsprint (the daily paper?) by treating in the same manner. Pleurotus ostreatus is a rapid grower when conditions are right. But there are many other fungi which also like the same sorts of substrates. Daniel B. Wheeler From geis.pa from pg.com Mon Sep 22 05:54:49 2008 From: geis.pa from pg.com (Geis, Phil) Date: Mon Sep 22 12:38:40 2008 Subject: [Mycology] RE: Mycology Digest, Vol 39, Issue 3 In-Reply-To: <200809181704.m8IH4mV02073@net.bio.net> References: <200809181704.m8IH4mV02073@net.bio.net> Message-ID: <3B4A11B0E75E524AB9EFDF2BDC5CE4F801ADA281@bdc-emb005.na.pg.com> As dry heat, 121C is insufficient but the 3 cycles of autoclaving on consecutive days is silly. Was this guy thinking of Tyndalization? A normal validated autoclave cycle will be sufficient. l Message----- From: mycology-bounces@oat.bio.indiana.edu [mailto:mycology-bounces@oat.bio.indiana.edu] On Behalf Of mycology-request@oat.bio.indiana.edu Sent: Thursday, September 18, 2008 1:05 PM To: mycology@magpie.bio.indiana.edu Subject: Mycology Digest, Vol 39, Issue 3 Send Mycology mailing list submissions to mycology@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/mycology or, via email, send a message with subject or body 'help' to mycology-request@net.bio.net You can reach the person managing the list at mycology-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Mycology digest..." Today's Topics: 1. Re: Mycology Digest, Vol 39, Issue 2 (Prakash Peralam Yegneswaran) 2. RE: Mycology Digest, Vol 39, Issue 2 (Geis, Phil) ---------------------------------------------------------------------- Message: 1 Date: Wed, 17 Sep 2008 09:46:59 +0530 (IST) From: Prakash Peralam Yegneswaran Subject: [Mycology] Re: Mycology Digest, Vol 39, Issue 2 To: mycology@oat.bio.indiana.edu Message-ID: <217884.79917.qm@web8902.mail.in.yahoo.com> Content-Type: text/plain; charset=utf-8 Please Find the answers as below the each questions ?? 1) Is the 121??C temperature sufficient to kill the Bacillus? Bacillus sps are notorious in forming endospores. Probably I think you might have encountered a thermophilic sps. It is sufficient to kill the vegetative form but not the resistant endospores 2) Is the autoclaving time cycle of 30 min. sufficient? ??Sufficient 3) How long must the autoclaving cycles be apart to make sure you kill the bacterial spores.? ?? 12 hrs 4) Is there any other methods I can use to kill the bacterial spores? ?? Autoclaving for 3 consequtive days with 12 hrs difference will help better in getting rid of the spores. ?? PRAKASH PERALAM YEGNESWARAN Faculty of Medicine, Lecturer in Microbiology, Incharge Medical Mycology Laboratory, Department of Microbiology, II Floor, Centre for Basic Sciences, Kasturba Medical College, Manipal University, Madhav Nagar, Manipal, Karnataka State, INDIA. ----------------------- Pincode : 576 104 Tel Off. : +91-820 2571201 extn. 22322 Mobile : +91-9886616153 Fax : +91-820 2571927 Email : prakashpy123@yahoo.co.in --- On Tue, 16/9/08, mycology-request@oat.bio.indiana.edu wrote: From: mycology-request@oat.bio.indiana.edu Subject: Mycology Digest, Vol 39, Issue 2 To: mycology@magpie.bio.indiana.edu Date: Tuesday, 16 September, 2008, 10:34 PM Send Mycology mailing list submissions to mycology@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/mycology or, via email, send a message with subject or body 'help' to mycology-request@net.bio.net You can reach the person managing the list at mycology-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Mycology digest..." Today's Topics: 1. asking for help (hardus.hatting@fabi.up.ac.za) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Sep 2008 15:20:33 +0200 From: hardus.hatting@fabi.up.ac.za Subject: [Mycology] asking for help To: "mycology@iubio.bio.indiana.edu" Cc: hardus.hatting@fabi.up.ac.za Message-ID: <48CFCEC1.28524.960AE5A2@hardus.hatting.fabi.up.ac.za> Content-Type: text/plain; charset=ISO-8859-1 Hi Paul I am battling with Bacillus bacterial contamination in autoclaved wheat and rice at the moment. I need to get the wheat/rice mixture 100% sterilized, but I think that this specific strain might be heat resistant. At the moment I am autoclaving the wheat/rice mixture at 121??C for 30 min. I will then incubate the flasks with the autoclaved wheat/rice mixture for 24 hours and will repeat the autoclaving again after 24 hours. This I will repeat three times, but to my disappointment it seems that the bacteria are not killed and after 1-2 weeks I will still loose allot of wheat/rice cultures due to bacterial contamination. Regarding the autoclave works 100% 1) Is the 121??C temperature sufficient to kill the Bacillus? 2) Is the autoclaving time cycle of 30 min. sufficient? 3) How long must the autoclaving cycles be apart to make sure you kill the bacterial spores.? 4) Is there any other methods I can use to kill the bacterial spores? I will really appreciate it if you can give me a few tips of how to get rid of this bacterial problem. Thanks allot Hardus ------------------------------ _______________________________________________ Mycology mailing list Mycology@net.bio.net http://www.bio.net/biomail/listinfo/mycology End of Mycology Digest, Vol 39, Issue 2 *************************************** Be the first one to try the new Messenger 9 Beta! Go to http://in.messenger.yahoo.com/win/ ------------------------------ Message: 2 Date: Wed, 17 Sep 2008 07:20:07 -0400 From: "Geis, Phil" Subject: [Mycology] RE: Mycology Digest, Vol 39, Issue 2 To: , Message-ID: <3B4A11B0E75E524AB9EFDF2BDC5CE4F801A76D01@bdc-emb005.na.pg.com> Content-Type: text/plain; charset="iso-8859-1" You could try gamma irradiation. -----Original Message----- From: mycology-bounces@oat.bio.indiana.edu [mailto:mycology-bounces@oat.bio.indiana.edu] On Behalf Of mycology-request@oat.bio.indiana.edu Sent: Tuesday, September 16, 2008 1:04 PM To: mycology@magpie.bio.indiana.edu Subject: Mycology Digest, Vol 39, Issue 2 Send Mycology mailing list submissions to mycology@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/mycology or, via email, send a message with subject or body 'help' to mycology-request@net.bio.net You can reach the person managing the list at mycology-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Mycology digest..." Today's Topics: 1. asking for help (hardus.hatting@fabi.up.ac.za) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Sep 2008 15:20:33 +0200 From: hardus.hatting@fabi.up.ac.za Subject: [Mycology] asking for help To: "mycology@iubio.bio.indiana.edu" Cc: hardus.hatting@fabi.up.ac.za Message-ID: <48CFCEC1.28524.960AE5A2@hardus.hatting.fabi.up.ac.za> Content-Type: text/plain; charset=ISO-8859-1 Hi Paul I am battling with Bacillus bacterial contamination in autoclaved wheat and rice at the moment. I need to get the wheat/rice mixture 100% sterilized, but I think that this specific strain might be heat resistant. At the moment I am autoclaving the wheat/rice mixture at 121?C for 30 min. I will then incubate the flasks with the autoclaved wheat/rice mixture for 24 hours and will repeat the autoclaving again after 24 hours. This I will repeat three times, but to my disappointment it seems that the bacteria are not killed and after 1-2 weeks I will still loose allot of wheat/rice cultures due to bacterial contamination. Regarding the autoclave works 100% 1) Is the 121?C temperature sufficient to kill the Bacillus? 2) Is the autoclaving time cycle of 30 min. sufficient? 3) How long must the autoclaving cycles be apart to make sure you kill the bacterial spores.? 4) Is there any other methods I can use to kill the bacterial spores? I will really appreciate it if you can give me a few tips of how to get rid of this bacterial problem. Thanks allot Hardus ------------------------------ _______________________________________________ Mycology mailing list Mycology@net.bio.net http://www.bio.net/biomail/listinfo/mycology End of Mycology Digest, Vol 39, Issue 2 *************************************** ------------------------------ _______________________________________________ Mycology mailing list Mycology@net.bio.net http://www.bio.net/biomail/listinfo/mycology End of Mycology Digest, Vol 39, Issue 3 *************************************** From Disa.Falke.3842 from student.uu.se Mon Sep 22 14:45:55 2008 From: Disa.Falke.3842 from student.uu.se (Disa Falke) Date: Mon Sep 22 16:24:37 2008 Subject: [Mycology] Choiromyces venosus Message-ID: <20080922214555.po044cq0js4w40c4@webmail5.uu.se> Hi! I am a Swedish student, doing my exam work on the truffle Choiromyces venosus. My exam work is a small part of a larger research project, where the pharmacognosy, systematics, ecology, cultivation possibility and vitamin content of Choiromyces venosus, will be investigated. Mainly I will examine how the ITS-sequence differs between specimens from different areas of Sweden (and Europe), but I will also do a literature study on chemical content, and cultural uses of the truffle. It has been hard to find any literature or research about the chemistry of the truffle, and my question is if anyone has any tips for me? I will be thankful for any information in the subject! /Disa Falke _______________________________________________ Mycology mailing list Mycology@net.bio.net http://www.bio.net/biomail/listinfo/mycology From dwheeler from ipns.com Tue Sep 23 03:16:59 2008 From: dwheeler from ipns.com (dwheeler@ipns.com) Date: Tue Sep 23 11:31:36 2008 Subject: [Mycology] Re: Choiromyces venosus References: Message-ID: <151a17ae-3caf-4b9d-a321-6028e2297cbf@s9g2000prg.googlegroups.com> On Sep 22, 12:45?pm, Disa Falke wrote: > Hi! > > I am a Swedish student, doing my exam work on the truffle Choiromyces venosus. > > My exam work is a small part of a larger research project, where the ? > pharmacognosy, systematics, ecology, cultivation possibility and ? > vitamin content of Choiromyces venosus, will be investigated. > > Mainly I will examine how the ITS-sequence differs between specimens ? > from different areas of Sweden (and Europe), but I will also do a ? > literature study on chemical content, and cultural uses of the truffle. > > It has been hard to find any literature or research about the ? > chemistry of the truffle, and my question is if anyone has any tips ? > for me? > > I will be thankful for any information in the subject! > > /Disa Falke > To my knowledge, Choiromyces venosus has not been found in the U.S. However, a close relative has been found. It is edible, and according to Charles LeFebre (current president of the North American Truffling Society) and especially esculent species. I believe the species name is C. alveolatus, but I could be wrong. Daniel B. Wheeler From dennis.thomsen from tryg.dk Tue Sep 23 03:19:03 2008 From: dennis.thomsen from tryg.dk (Dennis Rugtved Thomsen) Date: Tue Sep 23 11:31:40 2008 Subject: [Mycology] Does anybody know this fungi ? Message-ID: This fungi has been observed at a cellar wall. Best regards Dennis Rugtved Thomsen