[Mycology] Re: Mycology Digest, Vol 39, Issue 2

Prakash Peralam Yegneswaran via mycology%40net.bio.net (by prakashpy123 from yahoo.co.in)
Tue Sep 16 23:16:59 EST 2008


Please Find the answers as below the each questions
 
1) Is the 121ºC temperature sufficient to kill the Bacillus?
Bacillus sps are notorious in forming endospores. Probably I think you might have encountered a thermophilic sps. It is sufficient to kill the vegetative form but not the resistant endospores

2) Is the autoclaving time cycle of 30 min. sufficient?
 Sufficient 

3) How long must the autoclaving cycles be apart to make sure you kill the
bacterial
spores.?
 
12 hrs

4) Is there any other methods I can use to kill the bacterial spores?
 
Autoclaving for 3 consequtive days with 12 hrs difference will help better in getting rid of the spores.
 




PRAKASH PERALAM YEGNESWARAN
Faculty of Medicine, Lecturer in Microbiology,
Incharge Medical Mycology Laboratory, 
Department of Microbiology, II Floor,
Centre for Basic Sciences, 
Kasturba Medical College, Manipal University, 
Madhav Nagar, Manipal, 
Karnataka State, INDIA. 
----------------------- 
Pincode : 576 104 
Tel Off. : +91-820 2571201 extn. 22322 
Mobile : +91-9886616153 
Fax : +91-820 2571927 
Email : prakashpy123 from yahoo.co.in

--- On Tue, 16/9/08, mycology-request from oat.bio.indiana.edu <mycology-request from oat.bio.indiana.edu> wrote:

From: mycology-request from oat.bio.indiana.edu <mycology-request from oat.bio.indiana.edu>
Subject: Mycology Digest, Vol 39, Issue 2
To: mycology from magpie.bio.indiana.edu
Date: Tuesday, 16 September, 2008, 10:34 PM

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Today's Topics:

   1. asking for help (hardus.hatting from fabi.up.ac.za)


----------------------------------------------------------------------

Message: 1
Date: Tue, 16 Sep 2008 15:20:33 +0200
From: hardus.hatting from fabi.up.ac.za
Subject: [Mycology] asking for help
To: "mycology from iubio.bio.indiana.edu"
<mycology from magpie.bio.indiana.edu>
Cc: hardus.hatting from fabi.up.ac.za
Message-ID: <48CFCEC1.28524.960AE5A2 from hardus.hatting.fabi.up.ac.za>
Content-Type: text/plain; charset=ISO-8859-1

Hi Paul

I am battling with Bacillus bacterial contamination in autoclaved wheat and
rice at
the moment. I need to get the wheat/rice mixture 100% sterilized, but I think
that this
specific strain might be heat resistant. At the moment I am autoclaving the
wheat/rice
mixture at 121ºC for 30 min. I will then incubate the flasks with the
autoclaved
wheat/rice mixture for 24 hours and will repeat the autoclaving again after 24
hours.
This I will repeat three times, but to my disappointment it seems that the
bacteria are
not killed and after 1-2 weeks I will still loose allot of wheat/rice cultures
due to
bacterial contamination.

Regarding the autoclave works 100%

1) Is the 121ºC temperature sufficient to kill the Bacillus?
2) Is the autoclaving time cycle of 30 min. sufficient?
3) How long must the autoclaving cycles be apart to make sure you kill the
bacterial
spores.?
4) Is there any other methods I can use to kill the bacterial spores?

I will really appreciate it if you can give me a few tips of how to get rid of
this
bacterial problem.

Thanks allot
Hardus




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End of Mycology Digest, Vol 39, Issue 2
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