Antibody stuff

[Ross S. Chafetz]

Question


	I'm doing some antibody staining, using ABC
(vector kit).  The sections (35um) are free floating in wells.  Lately
my sections are sticking to themselves and to other sections (there are 7
per well, mice brains so they are pretty small).  I'm trying to pry stuck
sections apart with forcepts but that is also tearing the tissue.  If
anyone else has come across this problem, what did you do?  And I do
not want to do the staining on slides.

Thanks

Ross Chafetz

rchafetz at cephalo.neusc.bcm.tmc.edu