Antibody stuff

GLENN HOLM karuzis at wccf.mit.edu
Wed Aug 5 19:03:00 EST 1992


In article <1992Aug5.144701.21487 at wam.umd.edu>, wmsaidel at wam.umd.edu (William M. Saidel) writes...
>In article <13824 at gazette.bcm.tmc.edu> rchafetz at cephalo.neusc.bcm.tmc.edu (Ross S. Chafetz) writes:
>>
>>
>>Question
>>
>>	I'm doing some antibody staining, using ABC
>>(vector kit).  The sections (35um) are free floating in wells.  
>Sticking sections, in my experience, is due to i) poor fixation and/or
>ii) mold in the storage medium. Tell us more about the conditions you
agreed.  also tris-based buffers seem to be worse than phosphate buffers
for stickiness, and the younger the brain, the worse the sticking.  i suspect 
it's the fixation.
------------------------------------------------------------------
|Glenn Holm                         Internet:karuzis at wccf.mit.edu|
|M.I.T Dept. of Brain + Cog. Sci.   Bitnet:karuzis at mitwccf       |
|Cambridge, MA 02139       "Real Neuroscientists don't do gels!" |
------------------------------------------------------------------



More information about the Neur-sci mailing list