Help on whole cell patch clamp

John Zempel jzempel at morpheus.wustl.edu
Wed Jul 14 20:56:45 EST 1993


xiaoz at gandalf.rutgers.edu (Xiao Zhang) writes:

>I am trying recording from a cell line of our lab. Although I have some experiences about PC 12 and cultured neuron, I found it is very difficult for me to get signal from this cell line. The problem is that it is very hard to break-in.I tried sucking by mouth or using a syringe, the result is that almost everytimes when I break the membrane, I loss the seal at the same time. Any suggestion on this matter would be very appreciated. 


Two things:

Try different glass.

Try using Fl in your internal solution.  While this may not be desirable, it
may get you started.

John Zempel
jzempel at morpheus.wustl.edu



More information about the Neur-sci mailing list