Help! (Immunohistochemistry)

nishir at ohsu.edu nishir at ohsu.edu
Wed Aug 24 12:26:52 EST 1994


In article <1994Aug23.233531.4030 at news.vanderbilt.edu> neeraj jain
<JAINN at ctrvax.vanderbilt.edu> writes:
>
>Hi
>
>I need tips on doing immunohistochemistry on sections of fragile tissues
>like embryonic brain.
>
>e.mail replys welcome.
>
>Thanks for your time.
>
>neeraj jain
>..............................................
>JAINN at ctrvax.Vanderbilt.Edu
>
1. Immersion fix; wash; equilibrate in 30% sucrose
2. Embed in tissue Tek; freeze; cut 10 micron cryostat sections
3. Post-fix section after drying on slide if your antibody will put up with it.
4. Be real gentle when changing solutions

Any other questions feel free to email.

Rae Nishi
Oregon Health Sciences University
Portland OR




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