Acetylcholinesterase

J. A. Kiernan jkiernan at julian.uwo.ca
Wed Aug 31 09:30:13 EST 1994



On 31 Aug 1994 dbwilson at uoguelph.ca (Daniel B Wilson) wrote:

I am currently working on the catalytic activities of AChE and was
wondering if anyone has assessed the recombinant AChE for the putative
peptidase activity.  Further, does anyone know if antibodies to G-2 and G-4
isoforms exist and if they can be purchased ?

Thanks a lot for the help,

Daniel B. Wilson

  ---------------------------------

  Although natural AChE was once thought to have peptidase activity
  (perhaps hydrolysis of substance P), it now seems that this is
  not so.  Here's a reference:

   Checler, F., Grassi, J., Vincent, J. P. 1994. Cholinesterases
   display genuine arylacylamidase activity but are totally devoid
   of intrinsic peptidase activities. Journal of Neurochemistry
   62:756-763.

  Would you expect an activity in the recombinant enzyme that isn't
  present in the real thing?
                                     John A. Kiernan
                                     Department of Anatomy
                                     Univ. of Western Ontario
                                     LONDON, Canada  N6A 5C1
                                     (519) 679-2111 e.6822
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