NO formation from SNAP

Jack Lancaster jlanca at lsumc.edu
Mon Dec 12 18:16:28 EST 1994


re: posting by Tao T. Wang "Nitric Oxide -- glutamate"

        I can't help with the time course of glutamate change but can say a
bit about the rate of NO formation from SNAP.

        Just like NO does *not* react with NAP to generate SNAP, so SNAP
will *not* simply break down to form NAP and NO. NO does not react with
thiols to form nitrosothiols; this reaction requires the transfer of a
nitrosonium group (NO+, the one-electron oxidized form of NO). Even in just
water, the liberation of NO from SNAP involves several possible mechanisms
and is even more complicated in cells (see, e.g., J. Chem. Soc. Chem. Comm.
p. 1758 (1993)). The same is true for other compounds like nitroprusside
(which can also liberate cyanide which may well contribute to the toxicity
of NP which is independent of NO). When using these and other NO donors it
is important to actually quantitate NO formation and to control for other
potentially damaging reactions such as nitrosation or (in the case of NP)
cyanide toxicity. There simply are no uniform rates for these NO donor
compounds The only exceptions are the new NONOates introduced by Larry
Keefer at NIH.

Jack R. Lancaster, Jr.
Dept. Physiology
LSU Medical Center
1901 Perdido St.
New Orleans, LA  70112
504-568-6171
FAX: 504-568-6158
jlanca at lsumc.edu




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