Luciferase detection

Enrico Tongiorgi enrico at genes.icgeb.trieste.it
Wed Nov 23 09:12:30 EST 1994


Hallo,

I have transfected HeLa cells with a vector having as a reporter gene
the Luciferase gene. Is it possible to add substrates (D-luciferine etc.)
to the medium in oder to detect the cells which express the reporter gene ?
Does cells survive this procedure ? Has anyone a protocol for this ?
I thank very much those who wants to help me by sending a mail to:
TONGI at SISSA.IT

Thanks from
Enrico (Trieste, Italy)



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