polylysine buffer

Dave Pataky pataky at bcu.ubc.ca
Tue Nov 29 18:10:41 EST 1994


Hey neuron culturists,

I have started to culture primary neurons from embryonic chick brain, and
would like to coat the wells with polylysine, then laminin.  I'm looking
for a recipe for borate buffer for making up the polylysine, as i've heard
this is the buffer of choice.  Anyone have such a thing, maybe even know
why use borate buffer?  Any tips or alternatives would be greatly
appreciated.  Thanks in advance,

-- 
Dave Pataky
Dept of Zoology, UBC
pataky at bcu.ubc.ca



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