Neuroscience antibodies

[Klas Kullander]

>Could someone help me in testing the following antibodies in
>immunohistochemistry on rat brain section?
>
>1. anti-substance P
>2. anti dopamine beta hydroxylase
>3. anti- somatostatin
>4. anti-nerve growth factor
>
>I would appreciate any help. Thanks.
>--
>
>_______________________________________________________________________________
>Sujay Singh                                                    sujay at netcom.com
>NETCOM, the West Coast's Leading Internet Service Provider.      (408) 554-8649


Hi Netters

Regarding antibodies 1-3 you can find plenty of information in Tomas
Hokfelts work (Karolinska Institute, Stockholm, Sweden). As to antibodies
against NGF we have tried 27/21 (Boehringer Mannheim) with poor results
mainly because NGF is expressed in the rat brain at low levels.

Cheers

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Klas Kullander
Department of Developmental Neuroscience        *     *
BMC, Box 587
Uppsala University                                <>
S-751 23 Uppsala
Sweden                                          \_____/

e-mail address: Klas at bmc.uu.se
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