carbocyanine dyes

Dennis Churchill dchurchi at ACS.UCALGARY.CA
Mon Oct 17 17:10:08 EST 1994


We are about to try using carbocyanine dyes (i.e. DiI, DiO) to label 
cocultured neurons from rat brain. Can anyone provide some advice on 
the use of these dyes on dissociated cells regarding preparation of 
stocks, incubation times, when to add dye during the isolation 
procedure....     Any tips from experienced users are welcome.
Thanks in advance.

DENNIS CHURCHILL
---------------------------------------------------------------
DCHURCHI at ACS.UCALGARY.CA   ph:(403)220-4488   fax:(403)283-2700
NEUROSCIENCE RESEARCH GROUP, DEPTARTMENT of MEDICAL PHYSIOLOGY
UNIVERSITY OF CALGARY, CALGARY, AB, CAN, T2N 4N1.






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