Q: Astrocytes in culture
u9106353 at muss.cis.McMaster.CA
Tue Oct 18 13:35:47 EST 1994
Hi. I'm an undergraduate working part-time in a neuroscience lab, and
have been experiencing some difficulty with astrocytes in culture.
Although I do not grow up the cultures myself, I am dependent on them for
my own project, and I can't seem to get the cells in a "happy" state. My
attemps to research culturing techniques have been futile because most
research publications don't give the methods in enough detail for a novice.
As I mentioned before, my cultures are set up for me. All I do is
harvest the cells after the assay condition and perform Northern blots
from the RNA I extract. The assay condition is applied only four hours
after trypsonization for splitting purposes, and I believe that this may
be the problem, since after harvesting 10 x 100mm diameter culture dishes,
the cell pellet I obtain is between 1 - 2 mm in diameter.
Can anyone give me advice as to what is an acceptable waiting period after
trypsonization? Thanks in advance.
Sebnem Kirat =-)
Biochemistry IV, McMaster University
u9106353 at muss.cis.mcmaster.ca
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