Seizures and death after ibotenate lesions
blackbur at mcmail.cis.mcmaster.ca
Tue Oct 25 11:21:04 EST 1994
What are the best ways of avoiding seizures and death following
I've been trying to do ibotenic acid lesions of the ventrolateral
striatum. Following a paper from Trevor Robbins' lab I've been injecting
0.6ul of 10 ug/ul ibotenate at AP +.2, Lat 3.2, DV -6.5. The rats are
initially anesthetized with ketamine and xylazine. Problem is that
most of the rats experience severe seizures starting about two hours
after surgery and die after a few hours or a day. I've been treating the
seizures as needed with .05 ml of 5mg/ml diazepam i.p.
Any suggestions how I could improve survival rate while still getting a
thorough lesion would be greatly appreciated.
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