Transfecting primary neuronal cultures

Matthew Frosch frosch at dsg.harvard.edu
Wed Apr 12 10:19:27 EST 1995


I am trying to transfect various reporter constructs into primary
cultures of neurons.  My reporter is lacZ, so the cultures can contain
a mixture of cell types since the assay will be direct staining of the 
cells.  I have tried calcium phosphate methods without success and am 
interested in other methods which might work.

Any advice or guidance will be greatly appreciated.

Matthew Frosch
Pathology, Brigham and Women's Hospital
Boston, MA

frosch at dsg.harvard.edu





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