Rat GFAP sequence

Martin Kennedy mkennedy at chmeds.ac.nz
Sat Apr 29 19:39:34 EST 1995


In article <Pine.A32.3.91.950427085847.24961A-100000 at umabnet.ab.umd.edu>, "Mary P. Remington" <mremingt at umabnet.ab.umd.edu> writes:
> I need the rat GFAP sequence.  I also need primers for RT PCR.  Are they 
> commercially available?  Does anyone have a reference for the use of rat 
> GFAP primers?  Thanks, Mary

Here is the sequence you wanted, found using a gopher search at gopher.nih.gov 
(they also have a WWW site and email server capability). I have no idea 
whether primers are commercially available for this gene.  
-- 
Cheers,

Martin

NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
NN   NNNN              Christchurch, New Zealand              ZZZZZZZ
		Phone (64-3)364-0880   Fax (64-3)364-0750


8<  =======================  cut here  ======================= >8
LOCUS       RATMRNASEQ   2693 bp ss-mRNA            ROD       06-JUN-1994
DEFINITION  Rattus norvegicus glial fibrillary acidic protein (GFAP) mRNA
            sequence.
ACCESSION   L27219
KEYWORDS    glial fibrillary acidic protein.
SOURCE      Rattus norvegicus (strain Sprague-Dawley) (library: lambda gt11)
            brain cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Chen,W.-j. and Liem,R.R.K.H.
  TITLE     Re-expression of glial fibrillary acidic protein rescues the
            ability of astrocytoma cells to form processes in response neuronal
            contact
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
COMMENT     NCBI gi: 497617
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Rattus norvegicus"
                     /strain="Sprague-Dawley"
                     /sequenced_mol="cDNA to mRNA"
                     /tissue_type="brain"
                     /tissue_lib="lambda gt11"
BASE COUNT      653 a    740 c    783 g    517 t
ORIGIN      
        1 ccaagatgga gcggagacgt atcacctctg cacgccgctc ctatgcctcc tccgagacga
       61 tggtcagggg ccatggtcct accagacacc tgggtaccat tccgcgcctc tccctgtctc
      121 gaatgacgcc tccactccct gccagggtag acttctccct ggccggggcg ctcaatgccg
      181 gcttcaaaga gactcgggcc agcgagcgcg cggagatgat ggagctcaat gaccgctttg
      241 ctagctacat cgagaaggtc cgcttcctgg aacagcaaaa caaggcgctg gcagctgagc
      301 tgaaccagct tcgagccaag gagcccacca aactggctga cgtttaccag gcagaacttc
      361 gggagctgcg gctgcgtctg gaccagctta ctaccaacag tgcccggctg gaggtggaga
      421 gggacaatct cacacaggac ctcggcaccc tgaggcagaa gctccaagat gaaaccaacc
      481 tgaggctgga ggcggagaac aacctggctg tgtacagaca ggaggcggat gaagccacct
      541 tggctcgtgt ggatctggag aggaaggttg agtcgctgga ggaggagatc cagttcttga
      601 ggaagatcca tgaggaggaa gttcgagaac tccaggagca gctggcccag cagcaggtcc
      661 acgtggagat ggatgtggcc aagccagacc tcacagcggc tctgagagag attcgcactc
      721 agtacgaggc agtggccacc agtaacatgc aagaaacaga agagtggtat cggtccaagt
      781 ttgcagacct cacagacgtt gcttcccgca acgcagagct gctccgccag gccaagcacg
      841 aggctaatga ctatcgccgc caactgcagg ccttgacctg cgaccttgag tccttgcgcg
      901 gcacgaacga gtccttggag aggcaaatgc gcgaacagga ggagcgccac gcgcgggagt
      961 cggcgagtta ccaggaggca ctcgctcggc tggaggagga gggccaaagc ctcaaggagg
     1021 agatggcccg ccacctgcag gagtaccagg atctactcaa cgttaagcta gccctggaca
     1081 tcgagatcgc cacctacagg aaattgctgg agggcgaaga aaaccgcatc accattcctg
     1141 tacagacttt ctccaacctc cagatccgag aaaccagcct ggacaccaaa tctgtgtcag
     1201 aaggccacct caagaggaac atcgtggtaa agacggtgga gatgcgggat ggcgaggtca
     1261 ttaaggagtc gaagcaggag cacaaggatg tgatgtgagg tgggcccagc tggcggccct
     1321 tgccatacag tgtgagggcc taaagctccc tcctcagata gtcttgtttg ctaggcccaa
     1381 ttcccatcca caccagtgct ccccttcctt ctgtttttat gcccacggct cggtcagtgc
     1441 ggagtctcat ggacggcaca gaccaccctg catctccaac taacaggata ttcaccccaa
     1501 aggggcaatc aggaggggag gacccccctc cccccagctg ggttagaact ggaagaaaga
     1561 ggaaagacag gggcagggag acttaacaaa tcccttcctt catccttgtt gttatggaaa
     1621 ccgttgccag agctggaggt ctctgggaac tggactttga gttttcatag gcatgctgga
     1681 gcaagacaaa cattcagaca gaaaggaaaa catcccgagg caaagaatct ctagccagag
     1741 gcctaggcat ctggaagaac cctgacgatg taggagtggg tagggcagac ttgctacctg
     1801 gaatggccac taaggcagtc ctgaagggcc cccctccgga gggatgaccc tcgtgtatcg
     1861 gccccactga gcagccctgc aggttgatgc cccacgagcc tgtgaaaact tggttcttgg
     1921 catgtggcag gctctatagc ataagtggag agggaaggtg tactggaggg tatagaggag
     1981 gctctctggc ccctaagtgt ggatgagtgc ggagaggggg gagcccagga aggctacccc
     2041 gctcaggctg caggggtgcc atggcggagg aaccggtgga gataacttgg acaatggagt
     2101 tggaagttgt aggcaactag ttacacttgg ctctgaatcc ttggaatcaa ggaaatgacc
     2161 tgttctctca aagacactga aacaggagag agggagtgca tccactgggc agggtacagg
     2221 cgcgtctcag ttgtgaaggt ctatcctggt tgctcagtcc caactgcgca tcaccctggg
     2281 cttctcaacc tgaaagagtc cacaaccatc cttctgaggc cctccatccc cacaaccact
     2341 agctgttgtt ctccaagcca agggccccat tccctttctt atgcatgtac ggagtatcgc
     2401 ctagacttta agcgtccatc ctgtttgaaa gtttgggaaa ctgaaccacg ttgtgttcaa
     2461 ccagcctggt gtggagtgcc ttcgtattag tgtaccctct cggaagctgg ttggtgggca
     2521 ggtgaggaag aaatggagct gaaagtgtcc cctcagttgt cctttcctcc ccctctaagg
     2581 tccctccctt tccccaggac atcgtacact cccccccttg tcacctctgc taaccttcag
     2641 agcagtactg tcacctttac tcactgggca gaaataaaga cagtgtcaga ggc
//



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