lars.thomsen at dkb.dk
Sun Apr 30 04:41:00 EST 1995
JC>I would like to hear from anyone with experience with a
JC>microelectrode drive based on a high speed piezoelectric device
JC>mounted on a motorized 3D manipulator. The same device appears to be
JC>sold by different vendors (e.g., WPI has the MPM-10 Piezo Translator,
JC>and FST has the PM-10-1 one axis piezo-translator with motorized
JC>micromanipulator). Thanks in advance for any advice you might have.
Both WPI and FST makes very good micromanipulators I have tried the WPI
model but I wouldn't hesitate to buy the MPM-10 from FST. However the
minimum stepsize for the FST is 0.5 micrometer, which for my purpose is
I use a manually waterhydralic Narashigi MW-3. It took a while to
develop the "right way" to penetrate my neurones, but now everything is
working and with the waterhydralic you get a very stable electrode that
you can keep for hours in a cell.
One disadvantage of the manual micromanipulator is that you never know
exactly how deep you are in the tissue. If you are working with e.g.
wholemount of epithelia and you penetrate the cells without visual
control then it would be nice to know how deep you are in the tissue.
I am working with wholemounts of enteric neurones and I have mounted a
video on my microscope so I do not need sit and stare into the
microscope all the time. If you are going to work with penetrations
under visual control I would advice you to buy a waterdriven Narashigi
PLEASE NOTICE that the manual micromanipulator really is no good if you
try to penetrate single cells in culture, where the cells are not firmly
attached to the bottom of the dish. In that case I would buy a Piezo and
live with the 0.5 micrometer stepsize.
MSc Lars Thomsen 04-30-95 10.41
Royal Veterinary & Agricultural University
EMAIL : lars.thomsen at dkb.dk
FAX : +45 35 38 25 25
TLF : +45 35 28 25 13
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