NT-3 gene

[rey garcia]

Help! The coding sequence of the neurotrophin-3 gene is supposed to be 
in one exon. Is there actually another untranslated 5' exon? If so, is 
this conserved among mammals as well? Where can I get the sequences 
(I've tried the databases and I don't think they are there)? If that 5' 
exon exists, is conserved among mammals and the sequences are available, 
that will really help me a lot to design a new set of primers for my 
RT-PCR and easily rule out genomic DNA contamination. I don't want to 
DNAse treat my RNA samples as it makes my RNA more prone to degradation. 
Please e-mail any useful info to:

rey.garcia at stonebow.otago.ac.nz