About Pipette Puller

[Taosheng Liu]

Hi,

   Does anyone have experience with David Kopf vertical pipette puller?
I use a model 720 with a nichrome heating coil, but the coil always changes its
shape after several heatings so it's impossible to get the right electrdes. I'm
doing two-stage pulling to do whole-cell patch clamp.

   Any suggestions will be appreciated (it's unlikely for us to buy a new
puller). Please reply or e-mail me(liut at sacc.hscbklyn.edu)