About Pipette Puller

Gerald Pollack gpollack at bio1.lan.mcgill.ca
Tue Aug 22 10:06:44 EST 1995


In article <199508192354.QAA09527 at net.bio.net> LIUT%SNYBKSAC.BITNET at UBVM.CC.BUFFALO.EDU (Taosheng Liu) writes:
>From: LIUT%SNYBKSAC.BITNET at UBVM.CC.BUFFALO.EDU (Taosheng Liu)
>Subject: About Pipette Puller
>Date: 19 Aug 1995 16:54:43 -0700

>Hi,

>   Does anyone have experience with David Kopf vertical pipette puller?
>I use a model 720 with a nichrome heating coil, but the coil always changes its
>shape after several heatings so it's impossible to get the right electrdes. I'm
>doing two-stage pulling to do whole-cell patch clamp.

>   Any suggestions will be appreciated (it's unlikely for us to buy a new
>puller). Please reply or e-mail me(liut at sacc.hscbklyn.edu)

Kopf sells an attachment for this puller that holds platinum-ribbon filaments; 
these are much more stable than nichrome coils.




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