About Pipette Puller

chris elliott cje2 at york.ac.uk
Tue Aug 22 05:30:08 EST 1995


hello

Try using some platinum wire instead, and make a coil former out of a helical 
groove cut on a cylinder with the ends cut down to the diameter of the glass 
you use. then you can slip the former in and check the coil size

chris

In article <199508192354.QAA09527 at net.bio.net>,
   LIUT%SNYBKSAC.BITNET at UBVM.CC.BUFFALO.EDU (Taosheng Liu) wrote:
>Hi,
>
>   Does anyone have experience with David Kopf vertical pipette puller?
>I use a model 720 with a nichrome heating coil, but the coil always changes 
its
>shape after several heatings so it's impossible to get the right electrdes. 
I'm
>doing two-stage pulling to do whole-cell patch clamp.
>
>   Any suggestions will be appreciated (it's unlikely for us to buy a new
>puller). Please reply or e-mail me(liut at sacc.hscbklyn.edu)



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