osmiumtetroxide enhancement of immunocytochem.

Jane Q. Employee Employee_Q_Jane at lilly.com
Wed Aug 23 14:29:16 EST 1995


I have several DAB enhancement procedures I would be glad to share if 
you still are interested.  Please e-mail me and I will send them to you.

Karen Wightmanmdougher at math.rutgers.edu (Michael Dougherty) wrote:
>?
>I have done immuno against parvalbumin in brain tissue and staining is present
>but weak.  I've read that one can use osmium tetooxide to enhance visualisation
>of the precipitate from the DAB reaction.  However, I need specific instructions about how to use the osmium to do this.  If you can help me out, post in the
>news group or email me at kopl   that is kopello at eden.rutgers.edu.
>

-------------- next part --------------
From: mdougher at math.rutgers.edu (Michael Dougherty)
Newsgroups: bionet.neuroscience
Subject: osmiumtetroxide enhancement of immunocytochem.
Date: 16 Aug 1995 17:11:11 -0400
Organization: Rutgers University LCSR
Message-ID: <40tmtf$h53 at math.rutgers.edu>

?
I have done immuno against parvalbumin in brain tissue and staining is present
but weak.  I've read that one can use osmium tetooxide to enhance visualisation
of the precipitate from the DAB reaction.  However, I need specific instructions about how to use the osmium to do this.  If you can help me out, post in the
news group or email me at kopl   that is kopello at eden.rutgers.edu.



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