sucrose pretreatment for frozen sections

CROCKETTDP crockettdp at
Wed Aug 23 20:47:16 EST 1995

I have left adult rat brains and spinal cords in PBS buffered sucrose for
several days without any apparent problems.   I now tend to leave the
brains in 15-20% sucrose-4%-paraformadehyde solutions for up to several
weeks with no problems.   We are using the material for
immunocytochemistry and are antigens are very hardy.
David P. Crockett, Ph.D.
Department of Neuroscience and Cell Biology
UMDNJ-Robert Wood Johnson Medical School
675 Hoes Lane
Piscataway, NJ 08854

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