J. A. Kiernan
jkiernan at julian.uwo.ca
Mon Aug 28 15:43:54 EST 1995
In article <28AUG95.17062512 at nauvax.ucc.nau.edu> cwa at nauvax.ucc.nau.edu writes:
>I have a question concerning neurobiotin. I'm reacting with DAB; intensifying
>with Nickel using the Vector lab kit. The sections are air-dried, alcohol
>dehydrated, cleared with Fisher's hemo-de and mounted with PerMount.
>Within a week, all the staining will fade out completely.
The only questionable item in your procedure is the "homo-de"
clearing agent. What is it? If this is some trade-secret mixture
you should never use it in scientific research. Use xylene, which
certainly won't extract the DAB polymerization product.
John A. Kiernan
Department of Anatomy
Univ. of Western Ontario
LONDON, Canada N6A 5C1
e-mail: kiernan at uwo.ca
More information about the Neur-sci