Neurobiotin fading

cwa at cwa at
Mon Aug 28 12:06:25 EST 1995


I have a question concerning neurobiotin.  I'm reacting with DAB; intensifying
with Nickel using the Vector lab kit.  The sections are air-dried, alcohol
dehydrated, cleared with Fisher's hemo-de and mounted with PerMount.

Within a week, all the staining will fade out completely.  I have to photograph
them all quite soon, as the data is lost.

The Vector techs have never heard of it.  Does anyone have any suggestions to
my slides from fading ?


Curt Anderson
Northern Arizona University
Department of Biology
cwa at

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