Richard G. Wehby
rgw at neuron.uchc.edu
Wed Aug 30 13:34:40 EST 1995
In article <28AUG95.17062512 at nauvax.ucc.nau.edu> cwa at nauvax.ucc.nau.edu writes:
>I have a question concerning neurobiotin. I'm reacting with DAB; intensifying
>with Nickel using the Vector lab kit. The sections are air-dried, alcohol
>dehydrated, cleared with Fisher's hemo-de and mounted with PerMount.
>Within a week, all the staining will fade out completely. I have to photograph
>them all quite soon, as the data is lost.
>The Vector techs have never heard of it. Does anyone have any suggestions to
>my slides from fading ?
>Northern Arizona University
>Department of Biology
>cwa at nau.edu
The ethanols may be having an effect on the stability of the reaction
product. See Mesulam (1982) "Principles of horseradish peroxidase
histochemistry...", in Mesulam [ed]
_Tracing_Neural_Connections_with_Horseradish_Peroxidase_, John Wiley and
Sons, pp. 102.
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