Cell Body + Fiber Stain?
J. A. Kiernan
jkiernan at julian.uwo.ca
Thu Feb 9 09:54:43 EST 1995
In article <3hchrq$s5i at newsbf02.news.aol.com> bobiniv at aol.com (BobinIV) writes:
>From: bobiniv at aol.com (BobinIV)
>Subject: Re: Cell Body + Fiber Stain?
>Date: 9 Feb 1995 02:56:41 -0500
>Have you considered Kluver Barrera KV or the Auletta, both of which use
>the metachromatic properties of cresyl violet? Although the KV technique
>requires attention to detail and is time consuming, it gives excellent
>results. However, I've obtained good results with a relatively fast and
>easy staining technique described by Donovick, P. J. (A metachromatic
>stain for neural tissue., Stain Technology, 49: 49-51, 1974) using a
>combination of formalin and thionin. The cell bodies appear dark purple
>and the fibers are reddish pink.
The original formalin-thionin method was that of M.C. Chang
(1936) Anat. Rec. 65: 437-442. The reagent is used as a
combined fixative & stain, and the block is then embedded in
paraffin and sectioned. I tried it a long time ago, and it
worked first time, but it's not for anyone who's in a hurry
for the results!
It's important to realize that these methods stain only
the myelin (not axons) in the metachromatic pink-red colour.
Probably the metachromasia is due to sulphatide lipids. The
blue is, of course, from DNA and RNA.
John A. Kiernan
Department of Anatomy
Univ. of Western Ontario
LONDON, Canada N6A 5C1
(519) 679-2111 e.6822
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