Non stick brain slices
Jason Christopher Dugas
jcdugas at uclink.berkeley.edu
Thu Jan 5 17:55:33 EST 1995
Patrick Martin (patrick at anatomy.ucl.ac.uk) wrote:
: Does anyone have a nice recipe for a solution that could be used to stick brain
: slices to slides? I am currently using gelatin coated slides but when I dehydrate
: the sliced brains prior to staining they float off the slides with disconcerting
: The brains are from perfused rats, soaked in 30 % sucrose formalin to force out
: water, blocked, bonded to a post with tissue tek, and cut coronally at -10 degrees
: on a cryostat.
: Thanks in advance.
I've found that cryostat -generated slides stick nicely to Superfrost
slides (from Fisher), untraeted (use straight out of the box).
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