Non stick brain slices

Patrick Martin patrick at anatomy.ucl.ac.uk
Thu Jan 5 16:33:54 EST 1995


Does anyone have a nice recipe for a solution that could be used to stick brain
slices to slides?  I am currently using gelatin coated slides but when I dehydrate 
the sliced brains prior to staining they float off the slides with disconcerting 
regularity.  

The brains are from perfused rats, soaked in 30 % sucrose formalin to force out 
water, blocked, bonded to a post with tissue tek, and cut coronally at -10 degrees 
on a cryostat.

Thanks in advance.

Patrick 






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