RT-PCR from formaldehyde-fixed tissue??

Gregory E. Gonye PhD gonyege at lldmpc.dnet.dupont.com
Sun Jan 8 10:13:25 EST 1995


Hello,
We have access to a number of samples of tissue which has been previously
fixed by traditional means involving paraformaldehyde and/or gluteraldehyde.
Does anyone know if it is possible to obtain RNA from these samples suitable
for RPA or RT-PCR, or if IN SITU hybridization is possible.  Thanks for
responses in advance.    :-)



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