Non stick brain slices
Charles E. Weaver
wheat at mbcrrc.bu.edu
Thu Jan 12 11:26:16 EST 1995
In article <ace-1201950945520001 at 18.104.22.168>, ace at pop.nih.gov (Alex
> > Does anyone have a nice recipe for a solution that could be used to
> stick brain
> > slices to slides? I am currently using gelatin coated slides but when I
> > the sliced brains prior to staining they float off the slides with
> > regularity.
> > The brains are from perfused rats, soaked in 30 % sucrose formalin to
> force out
> > water, blocked, bonded to a post with tissue tek, and cut coronally at
> -10 degrees
> > on a cryostat.
> > Thanks in advance.
> > Patrick
We generally coat our slides 3x drying in our glass drying oven
between about 150 F.
I can't tell from your post but it sounds like you are not drying your
sections onto the slides properly the sections should be dryed onto the
slides on a slide warmer the heat helps to stick them onto the coated
slides (I leave them on overnight) if you are already doing this try
increasing the heat of the plate a little.
|| || __ __ __ __ __ ____ __ ______
|| || \ \ | | | | \ |
\\^// | | | |_ | |__ /__ \ |
\\^// / | | \ |
\\^// / / | | |__/ \ |
\\^// / / | | | \|
\\^// Charles E.Weaver
\\^// Boston University School of Medicine Phone (617)638-5323
| Department of Pharmacology L-603 Fax (617)638-4329
| 80 East Concord Street Boston MA 02118 cweaver at acs.bu.edu
More information about the Neur-sci