Non stick brain slices
dlrohwer at facstaff.wisc.edu
Fri Jan 13 06:53:52 EST 1995
In article <1995Jan5.213354.20749 at ucl.ac.uk>, patrick at anatomy.ucl.ac.uk wrote:
> Does anyone have a nice recipe for a solution that could be used to stick brain
> slices to slides? I am currently using gelatin coated slides but when I dehydrate
> the sliced brains prior to staining they float off the slides with disconcerting
> The brains are from perfused rats, soaked in 30 % sucrose formalin to force out
> water, blocked, bonded to a post with tissue tek, and cut coronally at -10 degrees
> on a cryostat.
> Thanks in advance.
You may try poly-L-lysine coated slides. I have used
these with great success for mouse and Drosophila. A bit more
expensive than gelatin, but I've never had a single section float.
Available from Sigma pre-coated or you can get the
pll and do it yourself.
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