in vitro ischemia

Dan Small bm189 at FreeNet.Carleton.CA
Wed Jan 18 15:37:14 EST 1995


I am trying to decide on a glucose concentration during an in vitro
ischemic insult to rat brain hippocampal slices.  Should I go from
10 mM to 2 mM, 10mM to 0 mM or 5 mM to 2 mM or 5 mM to 0 mM or
or just keep 4 mM right through and not drop the glucose at all?
Does anyone know the concentration of glucose in the brain in vivo
during a global ischemic insult?  Any help would be appreciated. Thanks.
Cheers,
Dan Small
Small at biologym54.lan.nrc.ca



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